https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27950 in vitro invasion assays showed that siRNA against KRT1 was able to reverse the DHA-induced inhibition of breast cancer cell invasion. In conclusion, KRT1 is involved in the anti-invasive activity of DHA in breast cancer cells.]]> Wed 11 Apr 2018 15:01:51 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18030 Wed 11 Apr 2018 14:49:14 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10728 0.05). There was greater motility of CD151-transfected vs. control cells, when transferring through migration chambers with or without matrigel-coated membranes (P<0.01, P<0.01). Fewer numbers of mutant-transfected cells were found on the membranes for both migration and invasion studies (P<0.01, P<0.01). CD151 knock-down PC3 cells showed decreased motility (P<0.01), but no change in proliferation (P>0.05). Our data show that CD151 does not change the proliferative properties of prostate cancer cells, but does promote migration and invasion, and suggest that CD151 plays a specific role in promoting prostate cancer cell motility.]]> Wed 11 Apr 2018 14:15:31 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14362 Wed 11 Apr 2018 12:28:38 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18034 1 cm, and e) c-KIT expression as valuable prognostic predictors for disease-free survival. We conclude that c-KIT expression is, apart from Breslow's depth, another valuable predictor of prognosis and survival. Lichen sclerosus may be associated with vulvar melanoma.]]> Wed 11 Apr 2018 12:14:22 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30091 Wed 11 Apr 2018 12:04:08 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26547 in vitro and in vivo. PI staining showed significant apoptosis in NPC cells accompanied by the overproduction of ROS and downregulation of mitochondrial membrane potential (MMP, ΔΨm) by 3-BrPA. However, the ROS scavenger N-acetyl-L-cysteine (NAC) significantly reduced 3-BrPA-induced apoptosis by decreasing ROS and facilitating the recovery of MMP. We elucidated the molecular mechanisms underlying 3-BrPA activity and found that it caused mitochondrial dysfunction and ROS production, leading to necroptosis of NPC cells. We investigated the effects of the caspase inhibitor z-VAD-fmk, which inhibits apoptosis but promotes death domain receptor (DR)-induced NPC cell necrosis. Necrostatin-1 (Nec-1) inhibits necroptosis, apparently via a DR signaling pathway and thus abrogates the effects of z-VAD‑fmk. In addition, we demonstrated the effective attenuation of 3-BrPA-induced necrotic cell death by Nec-1. Finally, animal studies proved that 3-BrPA exhibited significant antitumor activity in nude mice. The present study is the first demonstration of 3-BrPA-induced non-apoptotic necroptosis and ROS generation in NPC cells and provides potential strategies for developing agents against apoptosis‑resistant cancers.]]> Wed 11 Apr 2018 11:05:32 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14383 Wed 11 Apr 2018 09:49:12 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18028 Wed 11 Apr 2018 09:44:48 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32265 Tue 25 Jul 2023 12:50:05 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34397 in vitro. Notably, only the first eluted MP fraction bound HK₂ cells indicating a possible association between MP size and cell‑targeting properties.]]> Tue 03 Sep 2019 18:26:10 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33031 Tue 03 Sep 2019 17:55:22 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10449 Sat 24 Mar 2018 08:08:01 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30301 0.05). The inconsistency between results reported in the present study and other studies may be unique to the study population or be a result of the lack of a reliable standardized method for determining absolute sCD36 concentrations. However, further investigations are required to assess CD36 tissue expression in the study population and to assess the accuracy of various commercially available sCD36 ELISA kits. Thus, the availability of a standardized simple sCD36 ELISA that could be performed in any basic laboratory would be more favorable to the specialized flow cytometry methods that detect CD36⁺ microparticles if it was to be used as a biomarker.]]> Sat 24 Mar 2018 07:31:52 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27522 in vitro and in vivo models. Treatment with HDACi [suberoylanilide hydroxamic acid (SAHA)] and DNMTi [5-AZA-2' deoxycytidine (5-AZA-dc)] decreased cell proliferation in MiaPaCa2 cells, and SAHA treatment, with or without 5-AZA-dc, resulted in higher cell death and lower DNA synthesis compared to 5-AZA-dc alone and controls (DMSO). Further, combination treatment with SAHA and 5-AZA-dc significantly increased expression of p21^WAF1, leading to G1 arrest. Treatment with epigenetic agents delayed tumour growth in vivo, but did not decrease growth of established pancreatic tumours. In conclusion, these data demonstrate a potential role for epigenetic modifier drugs for the management of PC, specifically in the chemoprevention of PC, in combination with other chemotherapeutic agents.]]> Sat 24 Mar 2018 07:28:54 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24678 in vivo and to determine the underlying mechanism by which simvastatin reduces hepatic steatosis.]]> Sat 24 Mar 2018 07:10:52 AEDT ]]>